Plenary Lecture:
“Chemical Measurements In Vivo with the MasSpec Pen Technology”
Professor Livia Eberlin, University of Texas at Austin, Department of Chemistry
Student Lecture:
“Utilizing Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry (LC-IMS-MS) to Assess Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT)™ Derivatized N-linked Glycans in Biological Samples”
Karen Butler, Laboratory of Professor Erin Baker, North Carolina State University, Department of Chemistry
Glycosylation is a ubiquitous co/post-translational modification involved in the sorting, folding, and trafficking of proteins in biological systems. Perturbations in glycosylation have been implicated in a variety of disease states, including neurodegenerative diseases and certain types of cancer. The structure-function relationship between a glycan and its biological role is often difficult to discern given the complexity of these analytes. Efforts to further this research are often hampered by a dearth of analytical techniques that provide information about the three-dimensional conformation of glycans in addition to their composition. In this work, nano liquid chromatography-drift tube ion mobility spectrometry-mass spectrometry (nLC-DTIMS-MS) was combined with the Individuality Normalization when Labeling with Isotopic Glycan Hydrazide Tags (INLIGHT™) strategy to label and analyze a series of glycan standards and glycans enzymatically released from the glycoproteins horseradish peroxidase, fetuin, and pooled human plasma. The use of both a natural (NAT) and stable-isotope label (SIL) in the INLIGHT™ strategy were advantageous in DTIMS-MS as they were mobility aligned and illustrated specific m/z and structural trend lines, facilitating the rapid identification of putative glycans in complex biological samples.